Figure 1 | Scientific Reports

Figure 1

From: Cyclosporin A ameliorates cerebral oxidative metabolism and infarct size in the endothelin-1 rat model of transient cerebral ischaemia

Figure 1

Experiment timeline. Schematic timeline of the 3-day experiment reiterated with two animals per trial. On Day 1, 34 adult Sprague Dawley rats were stereotaxically implanted with two cerebral guides in the left hemisphere and a femoral vein access. On Day 2, the awake and freely moving rats were equipped with microinjection and microdialysis probes and attached to a balanced swivel. Continuous cerebral microdialysis was commenced, and dialysates were analysed every 15 min. When baseline values had stabilised, focal transient ischaemia was induced through intracerebral ET-1 microinjection followed by a randomised i.v. dose of CsA (15 mg/kg NeuroSTAT) or placebo solution. Microdialysis was continued for 5 hours, and after 24 hours rats were sacrificed for histological infarct volume estimation. CsA = cyclosporin A, ET-1 = endothelin-1.

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