Figure 3
From: Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR
Resonance assignment of DGK. (a) Assigned residues mapped on the topology plot of DGK. The plot was created based on the DGK X-ray structure (PDB 3ZE5)10 and refined by CSI values obtained from chemical shifts (see Suppl. Fig. 6). 84% residues of DGK could be assigned by dipolar- and scalar-coupling based experiments. (b) Automated resonance assignment by ssFLYA confirms 91.5% of the backbone and 89.1% of all (backbone + sidechains) assignments obtained manually. Color code: green - assignment by ssFLYA agrees with the manual reference assignment within a tolerance of 0.55 ppm; red - assignment does not match with the reference; blue - assigned by ssFLYA, but not manually; black - assigned manually, but not by ssFLYA. Backbone and sidechain nuclei are shown. For branched sidechains, the relevant row is subdivided into an upper part for one branch and a lower part for the other branch.
