Table 3 Precision, recovery and reporting limit and signal-to-noise ratio (S/N) for each BA.
Bile acids | Intra-assayCVs* (%) | Inter-assayCVs** (%) | Recovery (%)1000 ng/mL | Reporting limit(RL) ng/mL | S/N instandard |
|---|---|---|---|---|---|
Unconjugated | |||||
CA | 3.2 | 2.6 | 142 | 5 | 84 |
α-MCA | 2.8 | 23.6 | 101 | 5 | 48 |
β-MCA | 2.9 | 17.3 | 114 | 5 | 85 |
ω-MCA | 2.2 | 18.8 | 128 | 5 | 81 |
CDCA | 3.4 | 38.3 | 103 | 5 | 14 |
DCA | 4 | 19.3 | 115 | 5 | 54 |
HDCA | 2.9 | 9.7 | 152 | 5 | 20 |
UDCA | 3.5 | 27.3 | 95 | 5 | 24 |
LCA | 8.9 | 10.6 | 117 | 50 | 16 |
Glycine- conjugated | |||||
G-CA | 3.4 | 4 | 137 | 5 | 47 |
G-LCA | 1.7 | 31 | 78 | 5 | 22 |
G-CDCA | 3.7 | 17.2 | 116 | 5 | 60 |
G-DCA | 4.1 | 25 | 108 | 5 | 14 |
G-UDCA | 2.7 | 27.6 | 103 | 5 | 19 |
Taurine-conjugated | |||||
T-CA | 3.6 | 14.7 | 120 | 5 | 16 |
T-α-MCA | 4.1 | 27.5 | 111 | 5 | 61 |
T-β-MCA | 4.1 | 17.2 | 106 | 5 | 51 |
T-UDCA | 3.7 | 20.2 | 108 | 5 | 34 |
T-CDCA | 3.9 | 39.5 | 90 | 5 | 37 |
T-DCA | 4.8 | 38.7 | 86 | 5 | 50 |
T-LCA | 10.2 | 22.7 | 124 | 10 | 14 |
