Table 4 Primer sequences used in qPCR studies.
Gene | Forward primer | Reverse primer | Amplicon length |
|---|---|---|---|
Reference genes | |||
Actb | GCTTCTAGGCGGACTGTTACTGA | GCCATGCCAATGTTGTCTCTTAT | 101 bp |
Actg | ACCAACAGCAGACTTCCAGGAT | AGACTGGCAAGAAGGAGTGGTAA | 76 bp |
Hmbs | GAAACTCTGCTTCGCTGCATT | TGCCCATCTTTCATCACTGTATG | 101 bp |
Hprt | CCTAAGATGAGCGCAAGTTGAA | CCACAGGACTAGAACACCTGCTAA | 86 bp |
Rpl13a | CCTGCTGCTCTCAAGGTTGTT | TGGTTGTCACTGCCTGGTACTT | 103 bp |
Ubc | AGGTCAAACAGGAAGACAGACGTA | TCACACCCAAGAACAAGCACA | 101 bp |
Target genes | |||
cyp8b1 | CAGGAAGTTCCGTCGATTTG | GGCCCCAGTAGGGAGTAGAC | 60 bp |
Nr0b2 | AAAGGACCAACCAATCTCCA | GGGAGTTAGTCTTTCCCATGAGT | 70 bp |
Nr1h4 | GAAAATCCAATTCAGATTAGTCTTCAC | CCGCGTGTTCTGTTAGCAT | 106 bp |
