Figure 2
Sema3E vaccine improves systemic glucose intolerance. (A–C) Food intake (A) (n = 7, 6), body weight (B) (n = 6, 6), and eWAT/BW ratio (mg/g) (C) (n = 14, 11) of mice administered KLH-conjugated Sema3E vaccine (Sema3E vaccine) or KLH alone (Con). (D) Visceral fat/BW ratio of the indicated groups determined by CT scanning (n = 8, 8). (E) Oxygen consumption (VO2) (n = 8, 8), CO2 production (VCO2) (n = 8, 8), respiratory exchange ratio (RER) (n = 8, 8), and energy expenditure (EE) (n = 8, 8) in the indicated groups. (F) Glucose tolerance test (GTT) in the indicated groups (n = 14, 15). Outliers were excluded by boxplot analysis. All remaining data were analyzed by the two-tailed Student’s t-test (A–E) or repeated measures (F). *P < 0.05, **P < 0.01. Values represent the mean ± s.e.m. NS = not significant.
