Figure 3
Characterization of immune response to Sema3E vaccine. (A) Peripheral white blood cell count (WBC) in mice administered KLH-conjugated Sema3E vaccine (Sema3E vaccine) or KLH alone (Con) (n = 6, 5) and maintained on a high fat diet (HFD). (B) Peripheral blood counts of lymphocytes (Lymph), neutrophils (Neut), monocytes (Mono), eosinophils (Eosino), and basophils (Baso) in the indicated groups (n = 6, 5). (C,D) ELISPOT assay showing production of IL-4 (C) or IFN-γ (D) by splenocytes harvested from mice fed an HFD and administered KLH-conjugated Sema3E vaccine (Sema3E vaccine). Splenocytes (106 cells) were stimulated with KLH, recombinant Sema3E protein (r-Sema3E), or PBS (Con). Right panels indicate the number of spots per 106 cells in the indicated groups (n = 6, 6, 6 for (C), n = 6, 6, 6 for (D)). No outliers or abnormal values were excluded by boxplot analysis. All data were analyzed by the two-tailed Student’s t-test (A, B) or 2-way ANOVA followed by Tukey’s multiple comparison test (C,D). *P < 0.05, **P < 0.01. Values represent the mean ± s.e.m. NS = not significant.
