Figure 1 | Scientific Reports

Figure 1

From: ALDH1A1 regulates postsynaptic μ–opioid receptor expression in dorsal striatal projection neurons and mitigates dyskinesia through transsynaptic retinoic acid signaling

Figure 1

Severe reduction of MOR1 expression in the postsynaptic striatal neurons of postnatal and adult Aldh1a1−/− mice. (A) Representative images show MOR1 (green), ALDH1A1 (purple), CALB1 (blue) and TH (red) co-staining in the sagittal sections of 3–month–old Aldh1a1−/− and littermate Aldh1a1+/+ mice (n > 5 per genotype). Solid arrowheads indicate the MOR1–positive striosomes in the dorsal striatum (DS). Open arrowheads point to the ventral striatum (VS) and adjacent structures. Scale bar: 1500 µm. (B) Representative images show MOR1 (green), ALDH1A1 (purple), CALB1 (blue) and TH (red) co-staining in the coronal sections of 3–month–old Aldh1a1−/− and Aldh1a1+/+ mice (n > 5 per genotype). Arrows indicate the MOR1–positive striosomes in the dDS. Arrowheads point to VS. Dashed lines outline the segregation between DS and VS for image analyses. Scale bar: 1000 µm. (C) Scatter plot depicts the expression of Mor1 mRNAs in the DS of 3–month–old Aldh1a1+/+ (n = 4) and Aldh1a1−/− (n = 3) mice by qRT-PCR. Data were presented as mean ± SEM. Unpaired t test, t = 6.016, df = 5. **P < 0.01.

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