Figure 4

TRPM2 promotes cell migration and invasion through Akt-mediated EMT. (A) Western blot analysis of the protein level of phospho-Akt (Ser473), total Akt, phospho-PTEN (Ser380/Thr382/383) and total PTEN in AGS control and TRPM2-KD cells. (B) The protein level of phohpho-Akt before and 24 hours after treatment with 10 μM LY294002, a PI3K inhibitor (C) mRNA level of EMT markers before and 24 hours after Akt inhibition by LY294002 (D) Gap closure assay study of AGS wildtype cells at 0, 12 and 24 hours after LY294002 treatment (10 μM). The average results of three independent experiments were summarized in the corresponding bar graph. (E) In vitro analysis of migration and invasion ability of AGS cells with or without LY294002 treatment (10 μM) after 24 hrs; the number of migrated and invaded cells from three independent experiments are presented in bar graphs (t-test vs. Scr. ***p < 0.001; **p < 0.01; *p < 0.05).