Figure 1 | Scientific Reports

Figure 1

From: Identification of CD4 and H-2Kd-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein

Figure 1

IFN-γ ELISPOT assay of splenocytes from BALB/c mice immunized with pCAGGS-MCS BgQ1m. Overlapping peptides (5 µg/ml for each peptide) from glycoprotein Q1 of human herpesvirus 6B (BgQ1) were used for stimulation. Peptides were divided into two groups: the first group contained the first 24 peptides (P1-P24) (a) and the second group contained the other 24 peptides (P25-P48) (b). (c) Splenocytes from immunized mice were stimulated with the selected 12 peptides (5 µg/ml for each peptide). The results were expressed as spot forming cells (SFC)/1 × 106 splenocytes and the wells of “not reliably countable high signals” were defined as 300 SFC/1 × 106 splenocytes. Pep(-) indicates medium alone. The data are the mean ± SD of duplicate (a,b) and triplicated (c) wells. And the data are representative of three independent experiments. Independent experiments were carried out from independent mice.

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