Table 1 Optimized Parameters for CRI-3T3.
From: Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
Experimental Parameters | Optimal Values |
|---|---|
Cell number (per well) | 6000 |
Cell starvation time | 10–30 m |
Initial concentration of rhEGF | 10 IU/mL |
rhEGF dilution multiple | 4 |
rhEGF stimulation time | 48 h |
EC50 | 0.2959 IU/mL |
