Figure 6

Inflammatory profile of ECs cultured in nanofilms. (a) mRNA levels of ICAM-1 and E-Selectin in ECs cultured on different substrates for 72 h. Expression of ICAM-1 and E-Selectin was normalized by GAPDH and expressed relatively to the levels before platting (n = 6). (b) mRNA levels of ICAM-1 and E-Selectin in ECs cultured on different substrates for 72 h and then activated with TNF-α (10 ng/ml) for 6 h, as evaluated by qRT-PCR. Results are expressed as fold increase relative to non-treated cells (n = 3). (c) Flow cytometry analyses of ICAM-1 expression in ECs before (dark blue) and after (red) treatment with TNF-α (10 ng/ml) for 24 h. Percentages shown were calculated based on the basal levels of ICAM-1 (n = 3). In (a,b) statistical analyses were performed by Kruskal-Wallis test followed by a Dunn’s multiple comparisons  test. *p < 0.5, **p < 0.1, ***p < 0.001, p**** < 0.0001. Results are mean ± SEM.