Figure 3 | Scientific Reports

Figure 3

From: Hyaluronic acid inhibition by 4-methylumbelliferone reduces the expression of cancer stem cells markers during hepatocarcinogenesis

Figure 3

Effect of 4MU treatment in HCC cells JHH6 and Huh7. (A) Morphological cellular changes after treatment (scale bar = 200 µm). (B) Dose-dependent cytotoxicity of 4MU with concentration ranging from 0.0–8.0 mM by MTT test. Concentration was shown as log[mM]. (C) Relative mRNA expressions of hyaluronan synthases HAS2 and HAS3, and hyaluronidases HYAL1 and HYAL2 after treatment of 0.5 and 2.0 mM of 4MU. mRNA expression was normalized to reference genes 18S and ACTB. Statistical analysis: *p < 0.05, **p < 0.01 compared to untreated control of each cell line (0.0 mM = 1.0) (D) Presence of cytoplasmic HA (scale bar = 20 µm).

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