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Figure 1

From: Primary neurons can enter M-phase

Figure 1

t1EK2 induces DNA synthesis in differentiated neurons. (a) Percentage of BrdU positive neurons expressing LacZ (Control) or t1EK2 at 1 (Control: n = 129; t1EK2: n = 153), 1.5 (Control: n = 201; t1EK2: n = 129), and 2 (Control: n = 161; t1EK2: n = 94) dpt (16–17 DIV). Control neurons never incorporate BrdU. Three independent experiments for each dpt were carried out. As control neurons never incorporated BrdU, statistical analysis was performed by comparing percent BrdU incorporation in t1EK2-expressing neurons with zero (***p < 0.001; one-tailed t test for each dpt). BrdU incorporation could not be assessed at 3 dpt due to widespread neuronal death of t1EK2-expressing neurons. (b) Representative confocal images of BrdU incorporation in a LacZ control neuron (1 dpt) and t1EK2-expressing neurons at 1, 1.5 and 2 dpt. Graphs represent mean ± s.e.m. Scale bar: 25 µm.

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