Figure 3
p53 tumor suppressor blockade rescues t1EK2-induced BrdU incorporation. (a) Percentage of BrdU positive neurons expressing LacZ/p53DN (Control) or t1EK2/p53DN at 1 (Control: n = 325; t1EK2/p53DN: n = 376), 2 (Control: n = 406; t1EK2/p53DN: n = 294), and 3 (Control: n = 312; t1EK2/p53DN: n = 204) dpt (16–18 DIV). Control neurons never incorporate BrdU. Three independent experiments for each dpt were carried out. As control neurons never incorporated BrdU, statistical analysis was performed by comparing percent BrdU incorporation in t1EK2/p53DN-expressing neurons with zero (***p < 0.001; one-tailed t test for each dpt). (b) Representative confocal images of BrdU incorporation of a LacZ/p53DN control neuron (3 dpt) and t1EK2/p53DN-expressing neurons at 1, 2, and 3 dpt. (c) Confocal images showing BrdU incorporation in t1EK2/p53DN-expressing neurons at 5 dpt. Arrows identify RFP positive neurons and arrowheads MAP2-negative (non-neuronal) cells. Graphs represent mean ± s.e.m. Scale bar: 25 µm.
