Figure 5
Differentiated neurons can undergo cytokinesis. (a) Time-lapse video frames of a neuron undergoing cytokinesis (Supplementary Video S9). There is a delay between the formation of the intercellular bridge and final abscission, suggestive of the presence of a chromatin bridge. (b) Confocal images of daughter neurons (evidenced by MAP2 immunostaining) in a, confirming that abscission was complete. One daughter neuron survived (right) while the other (left) displayed pyknotic nucleus (white arrow). (c) Time-lapse video frames of a neuron undergoing cytokinesis (Supplementary Video S10). (d) Confocal images of daughter neurons (evidenced by MAP2 immunostaining) in c, confirming that abscission was complete. The daughter neuron on the left is multinucleated, indicative of aneuploidy. (e–h) Confocal images of anillin immunolabelling. Anillin stains the cleavage furrow/midbody during neuronal cytokinesis (e,f; for 360° view of f see Supplementary Video S10). White arrows indicate intercellular bridge/midbody. See also Supplementary Fig. S2f–h. Anillin is recycled from the midbody in putative daughter neurons after completing abscission (g,h). (i) Confocal images of a pH3 positive neuron undergoing cytokinesis (for 360° view see Supplementary Video S11). The intercellular bridge is traversed by pH3 positive chromatin, evidencing the presence of a chromatin bridge. White arrows identify daughter neurons (g–i). Blue, red and green arrows represent 3D orientation (e–i). H2B: Histone H2B tagged with EGFP. Scale bars: 25 µm (a,c, RFP and H2B-EGFP stills), 5 µm (a,b, bottom panels), 25 µm (e–i).
