Figure 6
Neurons recover AIS integrity after M-phase exit without cell division. (a) AnkG signal per µm in t1EK2/p53DN/TOP2α-expressing neurons in different stages of the cell cycle (green) and in control neurons expressing LacZ/p53DN/TOP2α (blue) at the indicated time points after suppressing the G2 checkpoint with MK1775, normalized to neighboring RFP-negative/MAP2-positive neurons. All groups were treated with AZ3146 to abrogate the SAC 2.5 h after MK1775 treatment. A single experiment was carried out (**p < 0.01, *p < 0.05; Welch’s F test and two-sided Games-Howell post-hoc tests; Interphase: n = 9, Prophase: n = 7, Prometaphase to telophase: n = 9, Multinucleation 6 h: n = 8, Multinucleation 9 h: n = 10, Control: n = 10). (b) Confocal images of AnkG-immunolabelled neurons in interphase, prophase, and prometaphase to telophase, multinucleated neurons 6 and 9 h post transfection, and control neurons. High magnification of AIS (boxes) is shown for each case. Arrowheads identify RFP positive neurons and arrows their respective AIS when present. Graphs represent mean ± s.e.m. Scale bar: 25 µm.
