Figure 4
From: Forceful mastication activates osteocytes and builds a stout jawbone
Increased mastication results in the bone formation at the enthesis of masseter muscle without affecting the condyle. (a) Schematic of the coronal section of the mandibular bone indicating the region of interest. Area specified by the red square was histologically analyzed. (b) Representative histological images of the coronal section of the mandibular bone at the enthesis stained with hematoxylin and eosin. Scale bar, 50 μm. (c) Representative immunohistological images of the coronal section of the mandibular bone at the enthesis. Scale bar, 25 μm. Osteocalcin (green); and nuclei (blue). (d) Schematic of the lateral image of the mandibular bone indicating the region of interest. The sections were sliced along the blue line and the area specified by the red bar was analyzed. (e) Representative histological images of the section of the condyle by Safranin O staining. Scale bar, 50 μm. 2 sections per mouse and 4 mice each group were analyzed in the histological analyses.
