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Figure 1

From: Primary lung cancer samples cultured under microenvironment-mimetic conditions enrich for mesenchymal stem-like cells that promote metastasis

Figure 1

Physiological hypoxia and cell derived matrix enhance proliferation of stromal cell lines and are non-desmoplastic. (A) Schematic of cell derived matrix preparation from IMR90 fetal lung fibroblast cell line and characteristics of microenvironment mimetic conditions. (B) Phase contrast images of equal numbers WI38 fetal lung fibroblast cells grown for 72 hours in four environmental conditions (2D Normoxia, 2D Hypoxia, 3D Normoxia, and 3D Hypoxia). Scale bars are 100 μm. (C) Doubling time of WI38, IMR90, and bone marrow mesenchymal stem cell line hMSC following short term growth in different environments (Statistical analysis performed by ANOVA with Dunnett’s test for multiple comparison to 2DN, *p < 0.05, **p < 0.01, and ****p < 0.0001; data presented as mean ± S.E.M.). (D) Western blot analysis of hypoxia inducible factor 1 alpha (Hif1a), alpha smooth muscle actin (α-SMA), and cell cycle inhibitors (p21, p27) from WI38 and IMR90 after 72 hours growth in different environments. Cropped images in the figure are derived from full length blots presented in Supplementary Fig. S3.

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