Figure 3 | Scientific Reports

Figure 3

From: Primary lung cancer samples cultured under microenvironment-mimetic conditions enrich for mesenchymal stem-like cells that promote metastasis

Figure 3

3DH Patient derived stromal cell lines are mesenchymal and possess mesenchymal stem cell-like characteristics. (A) Western blot analysis of tumor resection 927 and its tumor derived cell lines, fetal lung fibroblast cell line IMR90, and lung adenocarcinoma cell line A549 (upper). Western blot analysis of additional tumor resections and their derived cell lines probing for expression of pan-cytokeratin (lower). Cropped images in the figure are derived from full length blots presented in Supplementary Fig. S4. (B) Immunophenotyping of 3DH tumor derived cell lines with commonly identified antigens associated with MSC (Y-axis: CD90, X-axes: CD105 (left) and CD73 (right)). (C) Quantification of Colony Forming Unit-Fibroblast (CFU-F) per 25 cells plated in 3DH tumor derived cell lines, a matched 2DN derived cell line (2DN 959), bone-marrow derived mesenchymal stem cell line (hMSC), and IMR90 after 16–18 days of growth. Statistical analysis performed by ANOVA with Tukey’s test for multiple comparisons to the indicated groups, *p < 0.05, and ***p < 0.001; data presented are mean ± S.E.M.

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