Figure 2 | Scientific Reports

Figure 2

From: Intestine specific regulation of pig cytidine-5′-monophospho-N-acetylneuraminic acid hydroxylase gene for N-glycolylneuraminic acid biosynthesis

Figure 2

Detailed characterization and analysis of the Pi promoter region. (a) Analysis of nucleotide sequences of the Pi promoter region. For the transcription factors to interact, putative binding sites as DNA sequences are underlined. The end point of 5′deletion mutants is indicated by arrows. The position ‘+1’ indicates the transcription start site of 5′pcmah-1. Thick underlines indicate the oligonucleotide DNA sequences for the EMSA experiment. (b) The 5′deletion analysis of the Pi promoter region of pcmah in IPI-2I cells. Transcription factors, which exist in the region and are indicated by black arrows, are represented. Statistic bars represent the mean ± SE obtained from three independent determinations. Differences in the fold value of luciferase enzyme activities were statistically analyzed using the Student t-test: *p < 0.05.

Back to article page