Table 1 DNA sequences of primers and probes used in this study.
Primers for 5′ Deletion mutantsa | |
|---|---|
Pi-1600(Bgl II-F) | ACAGATCTTGAAGGACAGCTTTGGCTCT |
Pi-1100(Bgl II-F) | ACAGATCTAAGGATAACCTGACCCCTTTGC |
Pi-700(Bgl II-F) | ACAGATCTTGAGGATGTGGGTTTGATCC |
Pi-542(Bgl II-F) | ACAGATCTTCGGATCTGACATTGCTG |
Pi-260(Bgl II-F) | ACAGATCTAAGTGTGTTGGCTTTGACCTG |
Pi-223(Bgl II-F) | ACAGATCTAGCTTGAACGGCTTAACCAAG |
Pi(Hind III-R) | ACAAGCTTCAGTATTTCCGTTGACACAG |
Primers for Direct mutagenesis b | |
Pi-SP1a MutF | TTGACCTGaaaGTaGGGGCTGGGTAGCTTGAAC |
Pi-SP1a MutR | TACCCAGCCCCtACtttCAGGTCAAAGCCAACA |
Pi-SP1b MutF | GGTGGGGGaTaaaTAGCTTGAACGGCTTAACC |
Pi-SP1b MutR | TCAAGCTAtttAtCCCCCACCCCCAGGTCAAAG |
Pi-SP1ab MutF | TTGACCTGGGaaTGaGaaCTGGGTAGCTTGAACG |
Pi-SP1ab MutR | CTACCCAGttCtCAttCCCAGGTCAAAGCCAAC |
Pi-Ets-1 MutF | TATGCCACAttggGCAGCCCT |
Pi-Ets-1 MutR | AGGGCTGCccaaTGTGGCATA |
Probes for EMSA b | |
Pi-SP1 | ACCTGGGGGTGGGGGCTGGGTAGCTT |
Pi-SP1 Mut | ACCTGGGGGTGGGGGaTaaaTAGCTT |
Pi-Ets-1 | TATGCCACAGGAAGCAGCCCT |
Pi-Ets-1 Mut | TATGCCACAttggGCAGCCCT |
