Figure 5
From: Cargo diffusion shortens single-kinesin runs at low viscous drag
Non-monotonic dependence of run length on viscous drag reflects a specific asymmetry in the motor’s load-detachment kinetics. Vertical dashed lines in run-length panels, the critical viscous drag force (0.2 pN, Fig. 2D) that separates the region where the effect of thermal diffusion dominates (left) from the region where the effect of viscous drag dominates (right). (A) Experimentally measured load-detachment kinetics12,13 (inset) give rise to a non-monotonic dependence of run length on viscous drag. Data are duplicated from Fig. 1A (blue scatter). (B,C) Symmetric load-detachment kinetics (left) cannot support a non-monotonic dependence of run length on viscous drag (right). (D) Asymmetric load-detachment kinetics with reduced sensitivity for assisting versus hindering load (left) also cannot support a non-monotonic dependence of run length on viscous drag (right). For all panels, run length (mean ± standard error of the mean; N = 1000 for each simulation condition) was normalized by the unloaded single-kinesin value.
