Figure 2
Surface and Stemness Marker Characterization. Presentation of stem cell characteristics: CFU assay, D5 present significantly more CFU compared to MUG-Mel1 and C8, representative pictures of three biological replicates (A); Migration behavior detected by xCELLigence, D5 (line green) presented a high migration potential compared to C8 (line red) and MUG-Mel1 (line blue) representative pictures of three biological replicates (B); Flow cytometry measurements of CD34, CD44, and CD271, D5 present the highest amount of CD271 positive cells and was measured in triplicates (C); detection of various stemness marker, D5 compared to C8 expresses a significant higher level of SOX-2, whereas no significant difference regarding c-Myc was detected (n = 3) (D).
