Figure 4

Src knockdown in the ARC area prevented the CFA injection-induced increases in the expression of Src and phosphorylated SFKs at Y416 in this area. The gel shown in (a) (from left to right) was loaded with lysates respectively prepared from ARC tissues of rats without any treatment (Naïve, left) and rats which received the intra-ARC infusion of sh-NC (sh-NC, middle) or sh-Src (sh-Src, right) but no CFA injection (No CFA). Lysates prepared from ARC tissues of rats (which received the intra-ARC infusion of sh-NC or sh-Src 7 days before) at days 1, 3, 7 and 14 after the CFA injection into the hind paw were respectively loaded in the middle (CFA/sh-NC) and right (CFA/sh-Src) two lanes of the gels shown in (b). Lysates prepared from ARC tissues of rats in the same experimental sets without any treatment (Naïve) were loaded in the left two lanes of the gels. Sh-NC: rats which received the intra-ARC infusion of sh-NC; Sh-Src: rats which received the intra-ARC infusion of sh-Src. Each group of blots was from a same filter, and stripped and successively probed with SFK-pY416 and GAPDH antibodies or Src and GAPDH antibodies as indicated. The original full length blots are shown in Fig. S7. The ratio of the band intensities versus that of GAPDH was calculated and then normalized to the ratio in naïve animals [ = 1, dashed line in (c)] for examining relative changes. Bar graphs show summary data (mean ± SEM) of relative changes. *p < 0.05, Dunnett’s post hoc test in one-way ANOVA in comparison with that of rats which did not receive the CFA injection (No CFA). ^#,##,###p < 0.05, <0.01, <0.001 Bonferroni’s post hoc test in two-way ANOVA in comparison between rats which received sh-Src versus sh-NC infusions in the ARC area; Values in brackets indicate the number of experimental repeats.