Figure 3

Effects of hesperidin and linarin on human Vδ1⁺ cells in PBMCs. (A) CFSE-labeled human PBMCs from a healthy donor were cultured in the presence of 100 µg/mL hesperetin, hesperidin, acacetin, or linarin or 0.01% DMSO for 14 days. CFSE expression (upper) and CD25 expression (lower) in Vδ1⁺ T cells (Vδ1), Vδ2⁺ T cells (Vδ2) and αβ⁺ T cells (αβT) in the PBMC population gated on CD3⁺ are shown. The shaded histogram indicates isotype controls. The experiment was repeated three times, and representative data are shown. (B) Statistical analysis was performed. The data are expressed as the mean + SEM of technical replicates in three independent experiments. *P < 0.05, ANOVA with Dunnett’s test.