Figure 3 | Scientific Reports

Figure 3

From: A Novel Lactate Dehydrogenase Inhibitor, 1-(Phenylseleno)-4-(Trifluoromethyl) Benzene, Suppresses Tumor Growth through Apoptotic Cell Death

Figure 3

PSTMB inhibits LDHA activity in Michaelis-Menten and Lineweaver-Burk plots. To check LDHA enzyme kinetics, purified recombinant human LDHA protein (10 ng) were incubated in buffer containing 20 mM of HEPES-K+ (pH 7.2), 30 μM of NADH with 0, 0.1, 0.5, 1 and 2 mM of pyruvate in the presence or absence of PSTMB (0, 0.1, 0.5 μM). for 10 min. The fluorescence of NADH was examined at wavelength of excitation at 340 nm and emission at 460 nm with spectrofluorometer. Michaelis-Menten curves (A) and Lineweaver-Burk plots (B) are shown to determine the inhibition mode of PSTMB. The values are averages of three separate experiments. The results are plotted as means ± SD.

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