Figure 2 | Scientific Reports

Figure 2

From: H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth

Figure 2

(a) Diagram showing the ubiquitin-proteasome system (UPS) and inhibitors targeting upstream components of the proteasome. (b) Knockdown of proteasome α7 subunit in H727 cells effectively induced cell death to a similar extent as observed in H23 cells (images taken 48 h post-transfection). Immunoblotting analysis was performed to verify the efficient knockdown of α7 in H727 cells. (c) Comparison of the sensitivity (IC50 values) of H727 and H23 cells to carfilzomib, bortezomib, MG-132, PYR-41 (an E1 inhibitor) and P5091 (an USP7/USP47 inhibitor). Data are shown as mean ± SD derived from a single non-linear regression based on n = 3–4 replicates per compound per concentration.

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