Figure 4
From: Establishment of Novel Murine Model showing Vascular Inflammation-derived Cognitive Dysfunction
Enhanced aortic infiltration of macrophages and microglial activation in hippocampus of middle-aged mice induced by abdominal aortic aneurysm (AAA). (A) Representative aorta and histopathological analysis of infrarenal aorta in 3-month- (young, Y) and 12-month- (middle-aged, M) old mice after sham operation (Sham) or angiotensin II (AngII) infusion with CaCl2 application (CaCl2 + AngII). (B) Quantification of infrarenal aortic diameter. (C) Immunofluorescent staining for macrophages (green: Mac3, blue: DAPI) and EVG staining. (D) There was an inverse correlation between infrarenal aortic diameter and quadrant time in the probe trial. (E) Micrographs depict immunofluorescent labeling for IBA1 and MHCII (green: Iba1, red: MHCII, blue: DAPI). (F) Number of cells expressing IBA1, a marker of microglia and macrophages, was increased in M mice by AAA induction. (G) The proportion of IBA1-positive cells expressing MHCII was also greater in the hippocampus of AAA-induced M mice. ***p < 0.001. Scale bar = 100 µm. n = 7 for Y [Sham], n = 10 for Y [CaCl2 + AngII], n = 7 for M [Sham] and n = 7 for M [CaCl2 + AngII] (A–D); n = 5 mice/group (E–G).
