Figure 4
From: Profibrotic epithelial phenotype: a central role for MRTF and TAZ
The MRTF inhibitor CCG-1423 suppresses the UUO-induced rise in renal TAZ mRNA and protein expression. (a–c) Whole kidney extracts were prepared from mice 3 days after sham or UUO surgery and analyzed for TAZ mRNA by qPCR (a) (mean ± S.D., n = 5/per group), or TAZ protein using Western blotting (b,c). Representative blots are shown from 3 animals (b). Protein levels were quantified by densitometry and normalized to tubulin (n = 5/per group) (c). (d,e) TAZ was visualized by immunohistochemical staining in kidney sections from sham, UUO or CCG-1423-treated UUO mice (d). Images were quantified by determining the number of pixels above the threshold under the various conditions, and expressed as fold change compared to sham. At least 10 fields were analyzed from 2 randomly selected animals in each group (e) (mean ± .S.E., n > 20/group). Bar 200 μm.
