Figure 6
From: Profibrotic epithelial phenotype: a central role for MRTF and TAZ
MRTF mediates tubular expression of TAZ, TGFβ1 and CTGF during UUO. Kidney sections from sham, UUO and CCG-1423-treated UUO mice were mounted on membrane slides and rapidly stained with H&E staining. The tubular epithelium was then isolated using laser capture microdissection. Approximately 200 tubular excisions were collected from each animal, and the obtained material was processed for qPCR to detect tubular TAZ (a), TGFβ1 (b), CTGF (c) mRNA levels. Note that mRNA levels of each of these genes increased significantly in the tubular epithelium during UUO, and this effect was significantly suppressed by MRTF inhibition (mean ± S.D. n = 5/per group).
