Figure 7
From: Profibrotic epithelial phenotype: a central role for MRTF and TAZ
CCG-1423 treatment reduces early fibrogenesis during UUO. To assess early fibrogenesis, kidney sections were stained for fibrillary collagen using picrosirius red (PSR), and for Collagen IV and α-smooth muscle actin (α-SMA) using the respective antibodies. Quantitative immunohistochemistry revealed a significant increase in the levels of all of these fibrosis markers, an effect that was abolished or mitigated by CCG-1423 treatment; 3–5 randomly chosen fields/animal were quantified in 2 animals in each group (mean ± S.E., n = 6–10/per group). Similar results were obtained by whole-specimen scanning using a Huron Technologies TissueScope scanner and the TissueStudio analysis software (not show).
