Table 1 Primers used in the study.
From: Profibrotic epithelial phenotype: a central role for MRTF and TAZ
ID | Sense | Antisense |
|---|---|---|
Murine MRTF-A | 5′-TTG TCC CAG CCT GGT TCT CCA-3′ | 5′-ATC TGC TGA AAT CTC TCC ACT CTG-3′ |
Murine MRTF-B | 5′CCC CAG CAG TTT GTT GTT CAG CACT CTT-3′ | 5′- GAT GCT GGC TGT CAC TGG TTT CAT CTT G-3′ |
Murine TAZ | 5′-GAC GAG ATG GAT ACA CCT GAA-3′ | 5′-GAA GGC AGT CCA GGA AAT CA-3′ |
Murine TGFβ1 | 5′-GAA GGG CCG GTT CAT GTC ATG-3′ | 5′-TGT GAC AGC AAA GAT AAC AAA CTC CAC-3′ |
Murine PDGF-B | 5′-CCC ACA GTG GCT TTT CAT TT-3′ | 5′-GTG AAC GTA GGG GAA GTG GA-3′ |
Murine CTGF | 5′- TTG TAA TGG CAG GCA CAG GTC -3′ | 5′- CGC ACA AGA ACC ACC ACT CTG -3′ |
Murine IHH | 5′-GCT CAC CCC CAA CTA CAA TC-3′ | 5′-GCG GCC CTC ATA GTG TAA AG-3′ |
Murine GAPDH (reference gene) | 5′- TGC AGT GGC AAA GTG GAG ATT-3′ | 5′-TTG AAT TTG CCG TGA GTG GA-3′ |
porcine TGFβ1 | 5′-CTG TGT CTG TCC ACC ATT CA-3′ | 5′- GGT CCA AGA TGC TCA GTA AGT-3′ |
porcine TGFβ2 | 5′-TTG ATG GCA CCT CCA CAT ATA C-3′ | 5′-TGT AGG AGG GCA ACA ACA TTA G-3′ |
Porcine CTGF | 5′-GTG AAG ACA TAC CGG GCT AAG-3′, | 5′-GAC ACT TGA ACT CCA CAG GAA-3′ |
Porcine PDGF-B | 5′-CAA GTG TGA GAC GGT GGT GG-3′ | 5′-GCG GGG CTG AAC AAT TAG AG-3′ |
Porcine IHH | 5′-CTT GCA GCG CTG GGT CAT-3′ | 5′-CGC TAT GAA GGC AAG ATC GC-3′ |
Porcine GAPDH (reference gene) | 5′-GCA AAG TGG ACA TGG TCG CCA TCA-3′, | 5′-AGC TTC CCA TTC TCA GCC TTG ACT-3′ |
