Figure 2
From: Direct Effects of Nicotine Exposure on Murine Calvaria and Calvarial Cells
Analysis of Target Nicotinic Receptors. (a) qrt-PCR analysis for target nicotinic receptors on murine suture and synchondrosis RNA isolated from postnatal day 15 pups after nicotine exposure. Gene expression for nicotinic receptors may be modulated with exposure. n = 3 per group. (b,c) Fold change in nicotinic receptor expression compared to control (no exposure) in tissue from the cranial base (b) and coronal suture (c) regions. (d) Representative immunohistochemical staining for target receptors identified in tissues of interest (suture and cranial base) in high dose exposed and control (Secondary Antibody Only). (e,f) Quantification of percent staining of each target. n = 4 per exposure, 2 males and 2 females from different litters. Data presented as mean ± standard error of the mean. *p < 0.05, ***p < 0.001.
