Figure 1

Animal models. (a) Schematic representation of 7 bp deletion (blue) in genome of Lrrk2 deficient rat line. A part of exon 2 (red) and intron II (black) are depicted for orientation. (b) Generation strategy for Lrrk2 knock-down mouse line carrying a shLrrk2 transgene inserted into the Rosa locus. (c) Western blot analysis of Lrrk2 expression in brain-, spleen- and kidney- protein extracts of Lrrk2 deficient rats and (d) Western blot analysis of Lrrk2 expression in different tissues (brain, spleen and kidney) of Lrrk2 knock-down mouse in comparison to wild-type using anti-LRRK2 MJFF#2 antibody. f: female; m: male.