Figure 2

TSA stimulated acetylation of histones in promoter region of the Prkcz gene, responsible for PKMζ synthesis, and downregulated the PKMζ expression in cultured cortical neurons. (a,b) Figures illustrate the enrichment of acetylated histone marks H3K9 (a) [F_(1,6) = 12,721; p = 0,012; n = 4/group] and H3K18 (b) [F_(1,4) = 22,013; p = 0,009; n = 3/group] in promoter region of Prkcz gene (downstream promoter, responsible for synthesis of PKMζ transcripts) in neuron cultures incubated with TSA for 19 h. Values are presented as % of Input (total amount). (c) Experimental timeline for detection of PKMζ mRNA levels in control cultures and cultures incubated with trichostatin A (TSA, 100 nM) for indicated time (hours). Gene expression was determined using a qPCR method and normalized to YWHAZ [Kruskal-Wallis (KW): H (7, N = 39) = 23,01684 p = 0,0017; *p < 0,001; n = 4–6/group]. *Significant differences.