Figure 3
GlcNAc6ST3 in oligodendrocytes is a major sulfotransferase of R-10G-reactive KS in the cerebral cortex of adult mice. (a,b) Expression of the R-10G KS epitope in the 1% Triton-soluble fractions prepared from the cerebral cortex of adult WT mice and GlcNAc6ST1, 2, 3, and 4 single KO, and GlcNAc6ST1, 3 doubly-deficient (DKO) mice. The samples were pretreated with the chondroitinase ABC before western blotting. Representative results are shown (n = 3 in a, n = 2 in b). Densitometric quantitative analysis was used to measure the intensities of the bands in b (open arrowhead). The numbers denote average values relative to WT. N.D., not detected. ß-Actin was used as a loading control. (c) RNA-seq transcriptome results of the genes of each GlcNAc6ST family member in indicated cell types are shown. Cells were prepared from the cerebral cortex of adult mice49. Columns with mean values lesser than the threshold value are indicated by #49. Full-length blot images are presented in Supplementary Fig. S8.
