Figure 3
From: Circulating CD56bright NK cells inversely correlate with survival of melanoma patients
Functional characterization of CD56bright NK cells after stimulation (4 hours) with PMA/Ionomycin. (A) Histogram of the frequencies of CD56bright NK cells in patients and healthy donors. Patients included in subsequent functional experiments are indicated in red. (B) Representative dot plots of CCL4, IFNγ, GMCSF, CCL3 and TNFα after 4 hours of PMA/Ionomycin stimulation, and of perforin and granzyme B without stimulation of patient LAU627. (C) Histograms of IFNγ and CCL4 production (%) (CD56bright NK cells) in healthy donors and patients before and after stimulation. (D) Histograms of TNFα, GMCSF and CCL3 production (%) (CD56bright NK cells) in healthy donors and patients before and after stimulation. (E) Histograms of granzyme B and perforin (%) (CD56bright NK cells) in healthy donors and patients before stimulation. ns not significant *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
