Figure 5
From: Extensive conservation of the proneuropeptide and peptide prohormone complement in mollusks
Evolution of DH44-ELH peptide hormone families in lophotrochozoans, ecdysozoans, and deuterostomes. (A) Bayesian phylogenetic analysis using trimmed DH44/ELH protostomian sequences. Note the presence of three well-supported clusters: lophotrochozoan DH44, gastropod ELH, and ecdysozoan/deuterostome DH44/CRH. NCBI accession numbers, when available, are displayed after the species names. The newly described DH44/ELH sequences are available in Additional file 3. Branch support values correspond to posterior probability values. (B) Domain structure of DH44 and ELH sequences showing the signal peptide (blue box) and shared conserved motifs corresponding to the predicted amidated peptides (red boxes). (C) Multiple sequence alignment of ELH, DH44, and CRH bioactive domains in metazoans. Species names are abbreviated for convenience (e.g., Drosophila melanogaster = Dmel; Bombyx mori = Bmor). The conservation histogram corresponds to the number of conserved amino acid physico-chemical properties for each column of the alignment. The consensus displayed below the alignment is the percentage of the modal residue per column including gaps. (D) New evolutionary scenario of ELH/DH44 prohormone sequences within Mollusca. White circles correspond to the presence of motif repetitions within the precursor sequences.
