Table 1 Average concentration of target gene copies (copies/µl) across the three storage and isolation treatments, pre- and post-Inhibitor Removal Kit (IRK).

From: Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques

Treatment

Preservation buffer

eDNA Isolation

eDNA copies/µl pre-/post-IRK

A. Long-PCI

Longmire’s

PCI

0.6/927.8*

B. Long-CTAB-CI

Longmire’s

CTAB-CI

206.6/406.3

C. CTAB-PCI

CTAB

PCI

933.7/892.9

  1. (A) Long-PCI, Longmire’s storage buffer and phenol:choroform:isoamyl alcohol isolation, (B) Long-CTAB-CI, Longmire’s storage buffer and CTAB isolation and choroform:isoamyl alcohol cleanup, and (C) CTAB-PCI, CTAB storage buffer and PCI isolation. The Long-PCI treatment resulted in a significant increase (*) between the pre- and post-IRK treatment.
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