Table 2 Main effects of work shift, social jetlag (SJL) and their interaction for parameters of oxidative damage and antioxidant defenses, adjusted for age and working hours.

From: Night workers have lower levels of antioxidant defenses and higher levels of oxidative stress damage when compared to day workers

 

Night

Day

Shift

SJL

Shift*SJL

With SJL (n = 33)

Without SJL (n = 4)

With SJL (n = 25)

Without SJL (n = 17)

DF

p-value

DF

p-value

DF

p-value

TBARS (µmol/mg protein)

0.62 [0.59–0.66]

0.56 [0.49–0.65]

0.58 [0.54–0.61]

0.61 [0.57–0.66]

1

0.896

1

0.468

1

0.064

Carbonyls (nmol/mg protein)

2.79 [2.66–2.93]

2.51 [2.23–2.81]

2.74 [2.60–2.88]

2.79 [2.62–2.98]

1

0.288

1

0.237

1

0.080

H202 (µM H202)

32.5 [26.2–40.4]

24.5 [14.9–40.3]

26.2 [20.9–32.9]

18.7 [14.2–24.7]

1

0.204

1

0.051

1

0.867

Nitrite (nmol/mg protein)

1.74 [1.53–1.97]

1.64 [1.21–2.21]

2.02 [1.77–2.30]

2.05 [1.75–2.41]

1

0.089

1

0.823

1

0.684

FRAP (mM Fe+2)

0.78 [0.73–0.83]

0.77 [0.64–0.91]

0.95 [0.89–1.02]

0.95 [0.88–1.03]

1

0.002*

1

0.961

1

0.994

CAT (nmol/mg protein)

11.1 [9.7–12.6]

11.6 [8.1–16.7]

15.2 [13.2–17.4]

14.1 [11.7–16.8]

1

0.034*

1

0.951

1

0.570

SOD (USOD/mg protein)

610 [587–633]

610 [556–669]

712 [684–742]

697 [660–736]

1

<0.001*

1

0.915

1

0.900

GPx (nmol/min/mL)

12.8 [10.3–16.2]

14.4 [8.32–25.1]

8.4 [6.6–10.8]

11.9 [8.90–16.1]

1

0.085

1

0.126

1

0.571

  1. SJL was calculated based on the absolute difference between the average sleep time on working and rest days and was dichotomically categorized as >60 min (with SJL) or <60 min (without SJL).
  2. Abbreviations: TBARS, thiobarbituric acid reactive substances; carbonyls, plasma protein oxidation; H202, oxygen peroxide; FRAP, ferric reducing antioxidant power (total antioxidant capacity); CAT: catalase; SOD, superoxide dismutase; GPx, glutathione peroxidase.
  3. *p < 0.05 indicates statistically significant values. Data were represented as mean and Wald confidence interval (95% CI).