Figure 5 | Scientific Reports

Figure 5

From: Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles

Figure 5

ATG8 homologs accumulate in the vAC in their lipidated form. (A,B) Representative images of HFF cells infected with HCMV AD169 strain at MOI 0.5 for 4 days and immunostained for (A) GABARAPL1, and pp28 (viral tegument protein), GM130 or LC3; (B) GATE16 and pp28, GM130 or LC3 (NI: non-infected cells) (C) Immunoblot analysis of GABARAPL1 and GATE16 expression in HCMV-infected cells at the indicated times after infection. Autophagic flux was measured by addition of chloroquine (CQ) 4 h before lysis. Actin was used as a loading control. Full-length blots are presented in Fig. S7 (D) Confocal images of autophagy-deficient cells stably expressing ATG4B C74A infected with HCMV at MOI 0.5 for 4 days and immunostained for GM130 (Golgi) with LC3 or GABARAPL1 or GATE16. (*) non-transduced cells. Nuclei were subsequently stained with DAPI. Scale bars = 10 µm.

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