Figure 1

R. microplus tick endures H₂O₂ overexposure. H₂O₂ tolerance in ticks was evaluated measuring biological parameters after H₂O₂ injection in partially fed females. (A) Representative images of ticks after completed oviposition. Ticks were collected from groups injected with increasing amounts of H₂O₂ (0.5–5 μmol). (B) Tick weight gain was used to determine blood feeding capacity; (C) Tick reproductive efficiency index was used to determine the oviposition capacity; and (D) Eclosion rate, after H₂O₂ injection followed by completed engorgement by capillary tube blood feeding. Groups of 33 females were used for treatment at 0, 0.5, 1 and 2 µmol, and 17 females for each group treated at 3, 4 and 5 µmol. Data was verified for normal distribution using D’Agostino & Pearson normality test, statistical significance was assessed by Ordinary one-way ANOVA, being *p 0,0482, **p 0,0022.