Figure 2

Structure and functional analysis of MoDullard. (a) Domain composition of MoDullard. The gray bar represents an annotated Dullard-like phosphatase domain and the black bar indicates the section that was estimated to bind to chloramphenicol (Cm) using a T7 phage display method. (b) Conidial germination percentage and (c) appressorium formation percentage in ∆modullard and complementary strain. Each conidial suspension was treated with distilled water, 1% ethanol, or 300 µM Cm in 1% ethanol. **p < 0.01 compared with ∆modullard (Student’s t-test). (d) Conidial germination, germ-tube length, and appressorium formation percentage in MoDULLARD overexpression mutants in 300 µM Cm. Each percentage was assessed at 6 h post inoculation and was standardised against that of 0 µM Cm (control). *p < 0.05 compared with wild-type (Student’s t-test). Error bars indicate the standard error. The experiments were performed in triplicate for each sample and repeated three times.