Figure 3
Analysis of MoDullard orthologues in humans. (a) The domain composition of MoDullard homologues in humans: CTDSP1 (NP_067021.1), CTDSP2 (NP_005721.3), CTDNEP (NP_056158.2), CTDSPL (NP_001008393.1), and CTDSPL2 (NP_057480.2). The gray box indicates each Dullard-like phosphatase domain contained at the carboxyl terminus. (b) Appressorium formation percentage in human homologue complementary strains. Each conidial suspension was treated with distilled water. (c) Inhibitory effect of chloramphenicol (Cm) on CTDSP1 complementary strains. Each conidial suspension was treated with distilled water, 0.5% DMSO, or 300 µM Cm in 0.5% DMSO. *p < 0.05, **p < 0.01 compared with 0.5% DMSO (Student’s t-test). (d) Appressorium formation percentage of complementary strains. DxDxT/V motifs of mutMoDULLARD and mutCTDSP1 were inactivated. The appressorium formation percentage was assessed at 6 h post inoculation. *p < 0.05, **p < 0.01 compared with ∆modullard (Student’s t-test). Error bars indicate the standard error. The experiments were performed in triplicate for each sample and repeated three times.
