Figure 1
From: Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death
Expression of FUS in NP-derived astrocytes. mNPsc electroporated with plasmids allowing for the conditional expression of human WT-FUS were maintained for six days in the presence of astrocyte differentiation medium supplemented with or without doxycycline (Dox) and then fixed and stained. (A) Exogenous FUS, identified by an anti-FLAG antibody, was localized in the nucleus (WT-FUS) only in cells exposed to Dox. Similar staining profile was obtained by using an antibody recognizing both endogenous and the exogenous FUS (anti-FUS antibody). Scale bar 30 μm. (B,C) Representative blot and quantification of relative FUS levels at 48 and 72 hrs upon doxycycline stimulation. The bar graph shows a significant increase of FUS expression in induced cells (+Dox) compared to control cells (−Dox). The band observed in the lanes −Dox reflects endogenous FUS expression. Values are expressed as the mean of two independent experiments. FUS levels are normalized with respect to β-actin.
