Table 1 Inflammatory gene profiling of astrocyte-like cells expressing human WT- FUS.

From: Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death

 

up regulated

(fold change)

down regulated

(fold change)

Ccr2

 

0.27 ± 0.03

Cd38

15 ± 2.7

 

Csf2

11.9 ± 2.5

 

Cxcl10

 

0.5 ± 0.05

Fn1

4 ± 0.4

 

H2-Eb1

2.5 ± 0.3

 

Icos

2.5 ± 0.4

 

Il15

3.7 ± 0.5

 

Il5

4.4 ± 0.7

 

Il6

3.0 ± 0.4

 

Il7

2.9 ± 0.4

 

Lrp2

4.1 ± 0.6

 

Nos2

 

0.4 ± 0.1

Ptgs2

2.6 ± 0.4

 

Selp

 

0.5 ± 0.1

Tnf

4.5 ± 0.8

 

Nfatc4

3.4 ± 0.5

 
  1. mNPsc were differentiated into astrocyte cells for six days with or without doxycycline (Dox) and stimulated in the last 24 hrs with 10 ng/ml IL1β to assess the effect of FUS overexpression on astrocyte reactivity.
  2. The TaqMan Array 96-well Mouse Immune Response Plate from Applied Biosystems was used to determine the expression of 92 genes from immune system functions (the complete list is reported at the manufacturer website).
  3. Gene expression levels were normalized for four different genes included in the TaqMan Array, and reported as the fold change respect to the levels in cells that were differentiated in the absence of doxycycline and stimulated with IL1β. Values are mean ± SEM (number of independent experiments n = 3). Each sample of each experiment was obtained by collecting RNA form three parallel cultures. Only genes showing significant regulation, as analysed by unpaired Student’s t-test at P < 0.05 are shown.
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