Figure 6

Morphological changes and Sertoli-cell-only phenotype at E18.5 in SC-SF-1^−/− testes. Histological analysis of the testis stained with hematoxylin and eosin (H&E) in control (A) and SC-SF-1^−/− (D) mouse embryos at E18.5. H&E staining displayed loss of seminiferous tubules in SC-SF-1^−/− testis. Surviving tubules were mostly Sertoli-cell-only (SCO) lacking germ cells. Immunohistochemical staining of GATA4 in control (B) and SC-SF-1^−/− (E) testis. GATA4 staining shows the loss of cord formation in SC-SF-1^−/− testes. Immunohistochemistry of HSD3B (Leydig cell marker) in testes from control (C) and SC-SF-1^−/− (F) mice at E18.5. The Leydig cells were found to express HSD3B in SC-SF-1^−/− testes suggesting that loss of Sertoli cells did not affect Leydig cell function (scale bar = 250 μm). CON = control.