Figure 2
From: The Nuclear Receptor and Clock Repressor Rev-erbα Suppresses Myogenesis
Rev-erbα transcriptional regulation of the Wnt signaling pathway and cell cycle regulators. (A) KEGG pathway gene enrichment analysis of differentially expressed genes in Rev−/− vs. WT primary myoblasts. Genes that were significantly regulated with fold change ≥ 1.5 and adjusted P ≤ 0.05 from microarray analysis were analyzed (n = 3). Top 10 most significantly regulated biological pathways ranked by adjusted P values are listed. The dataset is deposited as NCBI GSE110765. (B) ChIP-qPCR analysis of Rev-erbα occupation of identified RORE sites in regulatory regions ±1 kb of candidate target genes in Wnt signaling or cell cycle regulators in proliferative C2C12 myoblasts (n = 3). Values are expressed as fold enrichment over IgG and known Rev-erbα binding site in Bmal1 first intron was included as positive control.
