Figure 6
From: The Nuclear Receptor and Clock Repressor Rev-erbα Suppresses Myogenesis
Activation of Rev-erbα by agonist SR9011 inhibits myogenic differentiation. (A–C) Inhibition of Rev-erbα agonist SR9011 on morphological progression of C2C12 myogenic differentiation for 6 days, as shown by representative phase-contrast images (A). Effect of SR9011 on myogenic differentiation as indicated by immunostaining of MyHC in day 3-differentiated C2C12 on chamber slides (B), with quantification of total MyHC+ and fused myofibers (C, n = 5/group) in the presence of vehicle (DMSO) or SR9011. DMSO (0.1%) or SR9011 (10 nM) were added 8 hours before myogenic induction and maintained throughout differentiation. (D) RT-qPCR analysis of SR9011 effect on suppressing myogenic gene expression at day 4 of C2C12 differentiation (n = 3). ***P ≤ 0.05 or 0.01 SR9011 vs. DMSO by Student’s t test.
