Figure 9
From: The Nuclear Receptor and Clock Repressor Rev-erbα Suppresses Myogenesis
Loss of Rev-erbα promotes satellite cell proliferative expansion during muscle regeneration. (A,B) Representative images of Pax7 immunofluorescent staining to identify total and EdU staining to label proliferative satellite cells, in day 8-regenerating TA post CTX injury (A). Arrowheads denote co-localization of Pax7 (red), EdU (green) with DAPI (blue) that identifies proliferating satellite cells. Phase contrast images of corresponding fields were shown in (B). (C–E) Quantification of number of Pax7+ satellite cells expressed as percentage of total nuclei (C), or normalized as the percentage of total number of myofibers (D); and the number of proliferative satellite cells as indicated by co-localized Pax7+ and EdU+ staining (E, n = 4). ***P ≤ 0.05 or 0.01 Rev−/− vs. WT by Student’s t test.
